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  Encyclopedia of Keywords > Net Charge > Isoelectric Point   Michael Charnine

Keywords and Sections
ESTIMATE
TECHNIQUE
GEL ELECTROPHORESIS
CONVENIENT WAY
EQUAL
MOLECULES
AMINO
POSITIVE CHARGE
PH VALUES
CHARGE
POSITIVELY
NEGATIVE CHARGE
NEGATIVE CHARGES
CATHODE
GEL
MOLECULAR MASS
PROTEINS
ELECTRICAL FIELD
ZERO
NET CHARGE
ISOELECTRIC POINT
Review of Short Phrases and Links

    This Review contains major "Isoelectric Point"- related terms, short phrases and links grouped together in the form of Encyclopedia article.

Definitions

  1. The isoelectric point (pI) is the pH of a solution at which the net primary charge of a protein becomes zero.
  2. The isoelectric point (pI) is the pH at which a particular molecule or surface carries no net electrical charge.
  3. Below its isoelectric point, the protein is positively charged and migrates towards the cathode.
  4. Thus, the isoelectric point is the value of pH at which the colloidal particle remains stationary in an electrical field.

Estimate

  1. If full-lenght protein sequence is available, it is possible to estimate the isoelectric point of the protein.

Technique

  1. The two dimensions that proteins are separated into in this technique correspond to isoelectric point and mass.

Gel Electrophoresis

  1. Gel electrophoresis, a technique used by scientists to separate molecules based on physical characteristics such as size, shape, or isoelectric point.

Convenient Way

  1. This is a convenient way to obtain the isoelectric point of a protein as well a to purify complex mixtures of proteins.

Equal

  1. Zwitterions in solution are only stable if the pH is equal to the isoelectric point.

Molecules

  1. While this pH gradent is being formed the protein molecules also migrate until they reach their isoelectric point.

Amino

  1. When this situation is present we sometimes indicate that this is the isoelectric point of the amino acid complex - a protein.
  2. This point is called the isoelectric point (pI) of the amino acid.

Positive Charge

  1. Thus, we have to increase the pH of the solution to remove positive charge in order to reach the isoelectric point.

Ph Values

  1. Anionic polyelectrolytes are used at pH values less than the isoelectric point.

Charge

  1. For some proteins, particularly large ones, the charge may be much less than -50 or much greater than +50 at pH's close to the isoelectric point.

Positively

  1. The positively charged amino acids were almost counterbalanced by negatively charged ones resulting in a calculated isoelectric point of 7.86.

Negative Charge

  1. At a certain pH known as the isoelectric point, the amine group has a positive charge (is protonated) and the acid group a negative charge (is deprotonated).

Negative Charges

  1. If positive and negative charges are both present in equal amounts, then this is the isoelectric point.

Cathode

  1. Define each of the following terms: amphoteric, ampholyte(s), isoelectric point, sodium dodecyl sulfate, cathode, and anode.
  2. A protein that is in a pH region below its isoelectric point (pI) will be positively charged and so will migrate towards the cathode.

Gel

  1. As a protein migrates down the gel, it reaches a pH that is equal to its isoelectric point.
  2. As shown above, each protein will "focus" at the point on the gel where the pH equals its isoelectric point, at which time it stops moving.

Molecular Mass

  1. The molecular mass of the enzyme is 17.7 kD with an isoelectric point of 4.2; glutamine is the N-terminal residue.

Proteins

  1. Separation of proteins may be by isoelectric point (pI), molecular weight, electric charge, or a combination of these factors.
  2. This technique separates proteins based on 2 properties: isoelectric point and molecular weight.
  3. Proteins can be separated according to their isoelectric point in a process known as isoelectric focusing.

Electrical Field

  1. In a solution with a pH above its isoelectric point, a protein has a nett negative charge and migrates towards the anode in an electrical field.

Zero

  1. Researchers can use gels to determine a protein's "isoelectric point," or the pH at which the protein's net charge is zero.

Net Charge

  1. When the net charge of an amino acid or protein is zero the pH will be equivalent to the isoelectric point: pI.

Isoelectric Point

  1. The proteins applied in the first dimension will move along the gel and will accumulate at their isoelectric point.
  2. Two-dimensional PAGE (2-D PAGE) differentiates proteins in the first dimension by isoelectric point and in the second dimension by molecular weight.
  3. Thus, point of zero charge at the surface is taken as equal to isoelectric point in the absence of specific adsorption on that surface.

Categories

  1. Net Charge
  2. Electrical Field
  3. Nature > Matter > Mass > Molecular Mass
  4. Molecular Weight
  5. Cathode
  6. Books about "Isoelectric Point" in Amazon.com

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  Originally created: April 04, 2011.
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