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Sequencing       Article     History   Tree Map
  Encyclopedia of Keywords > Genome > Sequencing   Michael Charnine

Keywords and Sections
GENOME SEQUENCING PROJECTS
AUTOMATED SEQUENCING
WHOLE GENOME SEQUENCING
NUCLEOTIDE SEQUENCING
SEQUENCING REACTIONS
CANADA
PRIOR
INTEREST
QUESTION
RELATED
SITES
AIRCRAFT
HOMOLOGY
ACCURACY
MILESTONE
DELIVERY
RESEARCHERS
DEVELOPED
ADVANTAGE
PRODUCTS
STRATAGENE
ORDER
TAXONOMY
COMPLETION
ECONOMICAL
REPLIES
SENDER
PACKETS
DATA PACKETS
GENE EXPRESSION
MOLECULAR CLONING
ORGANISMS
DETECTION
REAGENTS
DETERMINING
STRAINS
STREPTOCOCCI
NETWORK
DATA LINK
SERVICES
BRAIN
MAPPING
NUCLEOTIDES
AMINO
ISOLATION
IDENTIFICATION
Review of Short Phrases and Links

    This Review contains major "Sequencing"- related terms, short phrases and links grouped together in the form of Encyclopedia article.

Definitions

  1. Sequencing was accomplished with both sense and antisense nested PCR primers; only the sense direction is shown.
  2. DNA sequencing is the process of determining the nucleotide order of a given DNA fragment, called the DNA sequence.
  3. DNA sequencing was carried out using an Applied Biosystems ABI377 automated sequencer. (Web site)
  4. DNA sequencing was done by simultaneous bidirectional sequencing with universal M13 primers on a Licor 4200 IR 2 DNA sequencer.
  5. DNA sequencing was used to define the circumscription of Rhynchospora consaguinea Kunth Complex (Araujo, PhD thesis, 2001).

Genome Sequencing Projects

  1. Main sources for DNA and RNA sequences are direct submissions from individual researchers, genome sequencing projects and patent applications.

Automated Sequencing

  1. Automated sequencing of DNA by the chain termination method; each of four different chain terminating bases has its own specific fluorescent tag.
  2. The canine TfR sequence was determined by using automated sequencing and then submitted to GenBank (accession no. (Web site)
  3. Owing to its greater expediency and speed, dye-terminator sequencing is now the mainstay in automated sequencing.

Whole Genome Sequencing

  1. In the near future, the price of genome sequencing may drop low enough for a whole genome sequencing of Thais to be feasible.
  2. However whole genome sequencing indicates that chlamydiae do have a full set of genes for the biosynthesis of peptidoglycan.
  3. As a first step, we will perform a pilot study with a whole genome sequencing of 14 HCC and their corresponding non-tumor liver samples.

Nucleotide Sequencing

  1. The cagA variable region was amplified by PCR with primers cag2 and cagA26R prior to nucleotide sequencing.
  2. Cloning and nucleotide sequencing of acyl-CoA carboxylase genes. (Web site)
  3. Nucleotide sequencing was performed in six patients and identified eight mutations including three mutations in one patient. (Web site)

Sequencing Reactions

  1. Very useful for sequencing reactions, which proceed in a 5' - 3' fashion (addition of nucleotides to templated free 3' ends of primers).
  2. Sequencing reactions of cloned fragments were carried out using a DNA sequencing kit (PE Applied Biosciences) and analyzed by an ABI310 automated sequencer.
  3. Offers iOligo software which designs oligonucleotides for PCR or sequencing reactions, and orders them directly from synthesis companies. (Web site)

Canada

  1. Most of the sequencing was performed in universities and research centers from the United States, Canada and Great Britain. (Web site)

Prior

  1. In this case, a cloning step should be introduced prior to actual sequencing of the fragments.

Interest

  1. The DNA of interest may be a plasmid insert, a PCR product or a fragment representing a gap when sequencing a genome. (Web site)

Question

  1. At present, the two predictions cannot be confirmed but data from genome sequencing projects on AMF [ 29] and chytrids will help in answering this question.

Related

  1. Cloning and sequencing of major capsid protein (mcp) gene of a vibriophage, KVP20, possibly related to T-even coliphages. (Web site)
  2. Remarkably, DNA sequencing of the two species has proven them to be more distantly related to each other than either one is to the Sei whale. (Web site)

Sites

  1. Only precise dating and sequencing of rodent remains from sites with faunal material from 10,000-3,000 B.P. in Near Oceania will resolve this question.
  2. C) The sites of cleavage by factor Xa and trMASP2 are identical as confirmed by N-terminal sequencing as described in the text. (Web site)

Aircraft

  1. They coordinate the sequencing of aircraft in the traffic pattern and direct aircraft on how to safely join and leave the circuit.

Homology

  1. The cDNA fragments are cloned and identified by sequencing and homology searches.
  2. DNA sequencing revealed high degree of homology with polyketide synthase genes. (Web site)

Accuracy

  1. Other methods of DNA sequencing may have advantages in terms of efficiency or accuracy.

Milestone

  1. The sequencing of the mouse genome is a major milestone in modern biology as it is the only mammalian genome, other than human, to have been sequenced. (Web site)

Delivery

  1. There is a host to host synchronization mechanism and packet sequencing to provide duplicate detection and ordered delivery to the user process. (Web site)
  2. Because a TCP connection is not used, L2TP uses message sequencing to ensure delivery of L2TP messages. (Web site)

Researchers

  1. This allowed researchers to study the sequencing of DNA as it appears in practical diagnostic problems.
  2. Provider of software solutions to meet the needs of researchers seeking to extract information from genome sequencing projects and DNA microarray screening. (Web site)
  3. Researchers have identified the cause of Charcot-Marie-Tooth neuropathy using whole-genome sequencing.

Developed

  1. Variations of this method have been developed for automated sequencing machines.
  2. It is likely that the centromeres and telomeres will remain un-sequenced until new technology is developed that facilitates their sequencing. (Web site)
  3. Cloning and sequencing have been accomplished only with rotavirus, but PCR techniques are being actively developed for the other agents. (Web site)

Advantage

  1. The major advantage of this method is that the sequencing can be performed in a single reaction, rather than four reactions as in the labelled-primer method.

Products

  1. The RT-PCR reaction products were subjected to automated cycle sequencing using the sense and antisense nested PCR primers to prime the sequencing reactions.
  2. Processing was analyzed in vitro by import assays with isolated mitochondria and in vivo by immunoblot and N-terminal sequencing of processed products.
  3. In addition, amplified products can be checked by conventional sequencing methods. (Web site)

Stratagene

  1. Csk point mutants were generated using QuikChange (Stratagene) in the parental plasmid and were confirmed by DNA sequencing.

Order

  1. We are cloning and sequencing both cagA genes in order to further investigate this phenomenon. (Web site)
  2. Sequencing technology The instrumentation and procedures used to determine the order of nucleotides in DNA.
  3. Sequencing Determination of the order of base sequences in a DNA molecule. (Web site)

Taxonomy

  1. In recent years, the methods most widely used for taxonomy at the species level have been sequencing and electrophoretic methods.
  2. According to newer insights based on DNA sequencing, the taxonomy of the Gastropoda must be rewritten in terms of strictly monophyletic groups. (Web site)

Completion

  1. The completion of sequencing of the genome of Arabidopsis thaliana (AGI, 2000) marks a major milestone in plant biology research. (Web site)
  2. The completion of DNA sequencing at the different human genome centers is also dependent on gel electrophoresis. (Web site)
  3. DNA sequencing has become an essential tool for molecular diagnostics with the completion of many genome project.

Economical

  1. Specific goals include the development of sequencing and detection schemes that are faster and more sensitive, accurate, and economical. (Web site)

Replies

  1. SEQUENCING OF COMMANDS AND REPLIES The communication between the user and server is intended to be an alternating dialogue. (Web site)

Sender

  1. SEQUENCING OF COMMANDS AND REPLIES The communication between the sender and receiver is intended to be an alternating dialogue, controlled by the sender. (Web site)

Packets

  1. It does not guarantee delivery and does not provide sequencing of datagrams (packaged in packets). (Web site)
  2. This means that the arrival of datagrams is not guaranteed; nor is the correct sequencing of delivered packets. (Web site)
  3. UDP User Datagram Protocol is a connectionless protocol that does not guarantee the delivery or the correct sequencing of packets. (Web site)

Data Packets

  1. The session layer 270 protocols provide sequencing and synchronization of the dialog and data packets in a network connection. (Web site)
  2. The CMTS gateway agent examines all data packets at the application layer and stores connection state and sequencing information. (Web site)

Gene Expression

  1. Wang R F, Kushner S R. Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli.

Molecular Cloning

  1. Molecular cloning, sequencing, and expression of the genes encoding adenosylcobalamin-dependent diol dehydrase of Klebsiella oxytoca. (Web site)

Organisms

  1. The genomes represent both completely sequenced organisms and those for which sequencing is in progress. (Web site)
  2. The Quick Guide includes descriptions of these organisms and has links to sequencing centers and scientific abstracts. (Web site)

Detection

  1. Detection of p53 mutations was done by direct DNA sequencing. (Web site)
  2. Real-time DNA sequencing using detection of pyrophosphate release.

Reagents

  1. For example, chain-termination-based kits are commercially available that contain the reagents needed for sequencing, pre-aliquoted and ready to use.
  2. We also offer standard oligonucleotide synthesis and DNA sequencing services using Applied Biosystems instrumentation and reagents. (Web site)

Determining

  1. DNA sequencing: Determining the order of bases in a segment of DNA. (Web site)
  2. In genetics terminology, DNA sequencing is the process of determining the nucleotide order of a given DNA fragment.

Strains

  1. Sequencing of vacA and cagA genes revealed variable size, whereas the cagE gene was well conserved among strains. (Web site)
  2. These projects are sequencing the genome of a fellow primate (the marmoset) and identifying genetic variations in eight strains of rats. (Web site)
  3. All the monophasic isolates were positive for the fljB gene in PCR and sequencing of 10 strains revealed perfect match with the e,n,z15 group. (Web site)

Streptococci

  1. And information on streptococci has increased with the sequencing of the genome of four different species of streptococci. (Web site)
  2. We developed a new molecular tool based on rpoB gene (encoding the beta subunit of RNA polymerase) sequencing to identify streptococci. (Web site)
  3. Serotype identification of group B streptococci by PCR and sequencing. (Web site)

Network

  1. The context descriptor protocol used by the MT9088x family has no sequencing information, so the device cannot tell when packets are lost in the network.
  2. The network is not concerned with arrivals of groups of cells, sequencing or acknowledgment.
  3. End points are specifically identified, if required by the Network layer Sequencing. (Web site)

Data Link

  1. Note that the Internet Layer does not take advantage of sequencing and acknowledgment services that may be present in the Data Link Layer.

Services

  1. Services performed include session management, datagram service, data segmentation and sequencing, acknowledgment, and possibly routing across a WAN.
  2. Information on genomics projects including sequencing, mapping and services.

Brain

  1. Janata P, Grafton ST (2003) Swinging in the brain: shared neural substrates for behaviors related to sequencing and music.
  2. The angular gyrus is a complex part of the brain, responsible for things like body and space awareness, and logical sequencing. (Web site)

Mapping

  1. Genome projects: Research and technology development efforts aimed at mapping and sequencing some or all of the genome of human beings and other organisms.
  2. Fundamental to the myriad of genomic research efforts in operation around the world is the mapping and sequencing of whole genomes. (Web site)
  3. Here's a little key to help Nicholas Wade and other science writers differentiate between sequencing, decoding, deciphering, and mapping.

Nucleotides

  1. Sequencing: Determination of the order of nucleotides (base sequences) in a DNA or RNA molecule or the order of amino acids in a protein. (Web site)
  2. In genetics terminology, sequencing is determining the nucleotides of a DNA or RNA strand.
  3. Sequencing Determination of the order of nucleotides (base sequences) in a DNA or RNA molecule or the order of amino acids in a protein.

Amino

  1. The sequencing of the DHPS genes showed no mutations at amino acid positions 23, 60, 111, and 248 in this study (data not shown).
  2. Additional analytical data (e.g., sequencing, amino acid analysis) are available upon request. (Web site)
  3. Proteolytic processing may be detected by using antibodies specific for a cleaved product or by amino acid sequencing of the resistin protein. (Web site)

Isolation

  1. Isolation, hyperexpression, and sequencing of the aceA gene encoding isocitrate lyase in Escherichia coli. (Web site)
  2. Isolation of metagenomic DNA for PCR or next-generation sequencing.

Identification

  1. Nitrilase from Rhodococcus rhodochrous J1. Sequencing and overexpression of the gene and identification of an essential cysteine residue. (Web site)
  2. Identification of avian infectious bronchitis virus by direct automated cycle sequencing of the S-1 gene.
  3. Identification of vaccine candidates against serogroup B meningococcus by whole-genome sequencing.

Categories

  1. Genome
  2. Science > Biology > Genetics > Dna
  3. Science > Biology > Genetics > Cloning
  4. Pcr
  5. Encyclopedia of Keywords > Nature > Life > Genes

Related Keywords

    * Amplicons * Amplification * Analysis * Bases * Bioinformatics * Cdna * Celera * Chromosome * Chromosomes * Clones * Cloning * Complementary * Control * Data * Denaturing Gradient Gel Electrophoresis * Dgge * Dna * Dna Microarray * Dna Samples * Dna Sequencing * Edman Degradation * Electrophoresis * Entire Genome * Error Correction * Fig * Fragments * Gel Electrophoresis * Gene * Genes * Genetic Map * Genome * Genomes * Genome Sequencing * Genomics * Human Genome * Human Genome Project * Hybridization * Isolates * Mass Spectrometry * Method * Mitochondrial Dna * Mutagenesis * Mutation * Mutations * Organism * Pcr * Pcr Amplification * Pcr Products * Plasmids * Pneumocystis Carinii * Polyacrylamide * Polymerase Chain * Primer * Primers * Processes * Proteins * Proteomics * Restriction Digestion * Rt-Pcr * Samples * Sequence * Sequences * Sequencing Dna * Shotgun * Shotgun Sequencing * Tcp * Typing * Udp
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  Short phrases about "Sequencing"
  Originally created: June 19, 2008.
  Links checked: May 11, 2013.
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